14 Questions 4 Answers 0 Followers
Questions related from Daryl Goodrich
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
09 July 2024 9,144 2 View
I am trying to use a method from a paper by Do et al (https://www.sciencedirect.com/science/article/abs/pii/S0021967320311043?via%3Dihub) for a plant fingerprint. Short version: what could be...
01 June 2024 3,926 0 View
Hi there, adding a paper or towel to the tlc chamber is told to fasten the chamber saturation in TLC. But i could not find any information what paper can best be used for that - which works/which...
25 April 2024 7,460 4 View
I wanted to create the fingerprint of a phenol rich plant extract (dried powder, 15min ultra sound extraction in methanol) following the paper of Do et al (Complementary developing solvents for...
23 April 2024 9,988 4 View
Yeast from a small scale fermentation was heat inactivated and stored in a refrigerator after centrifugation - about 60-70% water content. The Yeast was planned for lipid/sterol extraction. Sadly...
08 February 2024 5,276 5 View
I want to recreate and extend an experiment from a paper and am not sure if my math is corrected. None of the authors is registered here. The authors did the following (text of the paper in...
02 October 2023 5,390 1 View
Bovine serum albumin has a mass of about 66kDa. I want to do binding studies of Bovine serum albumin with several secondary metabolites with a mass up to 2.5kDa. Can you recommend a filter -...
27 September 2023 3,181 4 View
Its been some time since i worked with enzymes. So as i now had a look at Enzymes by several vendors i wondered about the units the activity was given in and their meaning. e.g browsing Novozymes...
13 January 2023 8,263 2 View
Hi there, We use a UV366 and an UV254 Lamp for TLC. The UV254 is a Philips TUV 8W, still easily to get. The UV366 is a SterilAir BLB-8. Socket is G5, connection T5, 8W (The Lamp is about...
10 November 2020 8,745 2 View
Hi there, Is it possible to convert between color scales like Lovibond, Gardner, Apha or the solutions mentioned in Ph. Eur ? I found descriptions of spectophotometers where e.g. EBC and SRM were...
08 October 2020 6,039 1 View
I found several Articles - mostly in the topic of brewring and beer stability - mentioning a % Value of Catechin adsorption to PVPP. I browsed several artivles now - and could not find anyone...
25 November 2019 2,218 1 View
In most sources i found Loss on drying /LOD (w/w %)of a sample is determined by putting about 1g of a powdered sample into an oven at about 105°C for some hours. Afterwards it is placed in a...
24 October 2019 2,869 3 View
Hi, we have "inherited" an older UV-Vis spectrometer - a Cary 100 Bio - which is still available now from Agilent. As no one is experienced with this device i read available information i could...
01 September 2019 4,978 6 View
Hi, i have a fluid sample of a plant extract that over the weekend grew a massive contamination (lab bench - room temperature). So to find out which of the used ingredients (dry substances or...
17 April 2019 9,731 15 View
