I want to recreate and extend an experiment from a paper and am not sure if my math is corrected. None of the authors is registered here.
The authors did the following (text of the paper in quotation marks):
1.BSA solution:
"The BSA concentration was constant, 100 μM in the reaction mixture. BSA solution was prepared in pH 5 buffer (0.05 M acetate containing 50 μM ascorbic acid).
I found BSA having 66.430Da - so for e.g. 1L of 100µM my math is:
66.430 g/mol x 0,000100mol/L x 1L = 6,643g
So i would solve 6,643g BSA in the acetate-Buffer.
2.Samples:
"Sample solutions of 1.0 mM, 0.8 mM, 0.6 mM, 0.4 mM, 0.2 mM, and 0.1 mM
were prepared in MeOH/H2O (1/9, v-v)"
The Math should be the same as for BSA but with the molecular weight of e sample.
I am quite unsure with the ratios that are mentioned:
3."1/9, v-v "
I am unsure if this is the same as 1:9 and (v/v) - which I would read as 1 part A + 9 parts B. So e.g. 100ml MeOH + 900 ml Water
Finally a ratio of BSA + sample is mentioned:
4. "measured at sample/BSA ratios of 2:1, 3:1, 4:1, and 5:1."
Which would be the same for me as above (that is why i am confused) - e.g 3ml sample + 1ml BSA solution would be 3:1 for me.
How would you interpret/calculate those 4 points? Same as me - or do you see any error or something i interpret wrong?