Are quantitative surveys Inductive or Deductive?

I plan to do an online survey to gather responses from participants using a widely used stress scale. The results are either low, medium or high and out from this, I can infer as to which level of...

28 December 2020 7,307 6 View

Can I modify the Perceived Stress Scale (PSS) instrument?

I plan to use this tool for a quantitative study "proposal" to measure perceived stress. However, I am confused this tool because this tool (by Cohen) has ten questions that will assess stress "IN...

04 December 2020 8,268 4 View

Does anyone have any recommendations for transfecting a large plasmid into mammalian cells?

I'm trying to transfect a large plasmid (about 15 kb) into HeLa cells using Lipofectamine 2000. I've tried following the manufacturer's recommendations but can't seem to get much, if any,...

26 November 2020 5,764 4 View

Statistical Analysis for Online Survey Research?

I plan to conduct an online survey via Survey Monkey. It is a quantitative study that will measure perceived stress and I plan to use purposive sampling. No correlations, just a simple survey...

13 November 2020 936 3 View

What is the best Sampling Method for an Online Survey?

I wish to assess the level of stress among a specific group of nurses redeployed to other hospital settings (i.e, research nurses) during the COVID pandemic in my research proposal. May I ask for...

05 November 2020 4,671 3 View

The Perceived Level of Stress among a specific group of Nurses during COVID-19. Quantitative or Qualitative?

Hi. I want to create a research proposal assessing the perceived level of stress among a certain group of nurses during COVID-19. (For example, I might focus on Research Nurses who were deployed...

29 October 2020 8,815 10 View

How to convert color scales? Gardner to Lovibond to Apha to Ph. Eur ?

Hi there, Is it possible to convert between color scales like Lovibond, Gardner, Apha or the solutions mentioned in Ph. Eur ? I found descriptions of spectophotometers where e.g. EBC and SRM were...

07 October 2020 5,045 1 View

How to measure correctly on Agilent/Varian Cary 100? Zero/Baseline Blank/Reference- Single vs Double Beam?

Hi, we have "inherited" an older UV-Vis spectrometer - a Cary 100 Bio - which is still available now from Agilent. As no one is experienced with this device i read available information i could...

31 August 2019 4,698 6 View

Can you help me visually identify the mould genera on these contaminated samples?

Hi, i have a fluid sample of a plant extract that over the weekend grew a massive contamination (lab bench - room temperature). So to find out which of the used ingredients (dry substances or...

16 April 2019 9,612 15 View

Can any of the researchers tell me if he has used genetic algorithms in antenna geometry optimization problems, particularly phased arrays?

That one of the researchers from his point of view of his experience in the subject

10 December 2018 6,283 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View