I bought LP17 (Cat.No.: HY-P3400) from MCE. Their instructions recommend dissolving it with H₂O, adjusting the pH with HCl and ultrasonic treatment. How should I do it specifically?
I also found a contradiction:
The official website states that the maximum concentration of H₂O dissolution is 9.09 mg/mL, but it can be prepared into a 5 mM stock solution.
Then, according to "5 mg of peptide dissolved in 0.5099 mL of solvent", the concentration of LP17 reaches 9.8 mg/mL (exceeding the upper limit of the annotation).
Doesn't this exceed the upper limit of the solubility of the annotation?
Initial Dissolution: Add a portion of water, and vortex or sonicate to facilitate dissolution.
Acid Adjustment: Gradually add 1M HCl (5–10 μL per increment) with mixing until complete dissolution is achieved.
Volume Adjustment: Dilute to the final desired volume with water after dissolution.
Note: The experimentally determined maximum solubility is 5 mg/mL; concentrations should be optimized within this range.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
Dear Binbin Lee Do not take the exact numbers you find too seriously, they all depend on the various sources used.
Based on Heliquest https://heliquest.ipmc.cnrs.fr/cgi-bin/ComputParamsV2.py the peptide is predicted to be hydrophobic (see enclosed file).
Based on this it is reasonable to expect that DMSO is the preferred solvent. In H2O the net charge is zero (see also enclosed file which depicts the charge round pH 7), adjust the pH to 2-3 will make the peptide net positively charge which increase the solubility.
Best regards.
PS. If you want to learn more about how to interpreted Heliquest results, see for example
Article New User-Friendly Approach to Obtain an Eisenberg Plot and I...
Hongya Cheng, Rob Keller
That's awsome! Thank you, guys.
BTW, this article is really a nice job. Thanks a lot, Rob Keller .
Article New User-Friendly Approach to Obtain an Eisenberg Plot and I...
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