DAB Staining for yeast cells?

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Is it possible to dock more than one drug in the same active site or pocket of a protein to study synergistic/antagonistic/additive properties?

I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...

28 July 2024 7,413 6 View

Suggest a journal (Hybrid journal) which accepts short communications (Around 4 pages) and short reviews (Around 10 pages)?

In the area of material science and manufacturing engineering.

08 July 2024 5,576 0 View

What are the target genes used to detect biofilm formation by pseudomonas flouresence in dairy products ?

Detection the ability of pseudomonas flouresence to form biofilms in food

30 May 2024 8,050 1 View

How to calculate the succinate dehydrogenase (SDH) enzymatic assay ?

Hello everyone, Regarding the SDH assay calculation on a Microsoft Excel sheet, I need assistance. Using the UV spectrophotmetric approach, I conducted an in-vitro SDH experiment in the A549 cell...

21 May 2024 4,985 0 View

How to extract collagen from bones specimen?

how to extract collagen from bones specimen?

27 April 2024 6,307 0 View

Does anyone notice a pink stain along with blue stain when using trypan blue with single nuclei suspension as shown in the attached picture?

Hi there, I am using 0.4% trypan blue dye to count my nuclei suspension (generated from snap-frozen intestinal colon tissue). Although I expected a blue stain only (since nuclei resemble the dead...

17 April 2024 4,472 0 View

Which software can be used for opening tfh files for analysing the results?

I have got measurement results from China. But they have given me the results in tfh file format. Can anyone suggest a software to open the file and analyse the results?

14 April 2024 789 3 View

Why does community engagement play an important role in addressing challenges related to blood donation and transfusion services in Western Kenya?

This question is based on research done by Kavulavu et al. et al. in 2022, entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya." In this...

10 April 2024 9,791 0 View

Design of compact Battery Charger?

Description : 1. Input rating - Nominal 230V AC. Max 300V AC - Single Phase 2. Output rating - 60V, 15Amp max 3. Efficiency > 90%, 4. Max operating ambient temperature: 50 deg C 5. Volumetric...

02 April 2024 7,339 3 View

R2 falls when adding constructs in Amos?

In Amos, I am explaining a dependent construct with one exogenous construct and get a squared multiple correlation for the dependent of .48. I then add a second exogenous construct as an...

19 March 2024 7,255 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View