I am doing research on stroke animal models and generally use middle cerebral artery occlusion induced focal cerebral model. As you know the infarction degree is quantified by TTC staining and the brain slices are thrown after the photographing. I am suspecting that can we use the TTC stained brain section for other purpose such as protein extraction and western blot or RNA extraction etc. If you have any idea about it please let me know. Thanks in advance.
One of the research group used TTC brains for mRNA extraction and western blots, we tried to reuse it but could not reproduce same results, try not using formaldehyde to fix it may be you will be able to use those tissues for mRNA isolation. Please let me know if it works.
Hi Jatin
You report that One of the research group used TTC brains for mRNA extraction and western blots, can you please let me know that paper?
Dear Bhakta,
Apart from some histological stains we generally do not use TTC stained material for other methods,
Best, Clemens
Here is the link :http://www.sciencedirect.com/science/article/pii/S016502701000004X
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