How can we perform the skin sampling from psoriasis-like mice model for immunohistochemistry?

Hello everyone ! I am willing to sample the mouse skin for immunohistochemistry analysis from the mouse having psoriasis-like symptoms in dorsal skin layer. Could you please help me how should I...

07 August 2017 4,066 1 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

08 September 2016 6,826 1 View

How can we determine the phenotype of activated microglia through its morphology?

Hello ! I am working on microglia phenotype study following focal cerebral ischemic challenge in mice. Through the analysis of different biochemical markers (by qPCR, and western blot) we can...

08 September 2016 2,879 9 View

Is it okay to use water while diluting cDNA or primers?

Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. Recently we use DEPC water only for RNA work. For...

03 April 2016 3,781 5 View

Could you please suggest me the mounting medium/solution for cells ?

I am using VECTA SHELD mounting solution to mount my cells growing in coverslips. I wish to fix my cells using fixative solution and for that I have to use another mounting solution. And moreover,...

03 April 2016 9,588 2 View

Could you please let me know the size and length of monofilament for tMCAo induction in C57/BL6 male mice of 8 weeks?

I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...

02 March 2016 1,208 7 View

Does all experimental interventions are neuroprotective following stroke?

Could you please provide me some article which showed improvement of neurological deficit without change in infarct volume or vice versa after drug treatment flowing stroke induction in rodents.

02 March 2016 7,036 3 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

01 February 2016 7,414 2 View

How to extract proteins from formaldehyde fixed frozen tissue section (any kits) ?

I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...

10 November 2015 2,837 2 View

What would be the best marker for microglia in cerebral ischemia ?

We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...

09 October 2015 2,506 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View