17 Questions 8 Answers 0 Followers
Questions related from Ananya Chatterjee
If after activation jurkat cells lose viability then how can flow cytometry based assays or cell proliferation assays will be performed ?
30 April 2025 320 1 View
In literature SPR is generally done through ectodomain of protein. Can we use same ectodomain containing protein for performing ELISA (suppose we want to check of a particular compound's binding...
05 March 2025 8,486 0 View
Does resting (Culturing in T cell media) of T cells helps in proliferation of T cells? What should be the resting period?
21 February 2025 3,280 2 View
As we know that the expression of PD 1 is less in jurkat cell. To express PD1 jurkat cell need to be stimulated or activated. But there is a chance of activation induced apoptosis. How this...
14 February 2025 2,424 2 View
In FLISA or Fluorescence linked immunosorbent assay which type of 96 plates are used and which plate reader should we have to use?
05 February 2025 448 4 View
•Why the above mentioned ratios are maintained? is this ratio resemble the breast tumor microenvironment? can anyone suggest the coculture ratio of Jurkat/T cell : MCF-7 for any T cell mediated...
17 January 2025 4,767 0 View
Literature said it would be either 1:1 or 5:1 or some other ratios are also mentioned. what are the reason behind this ratio?
12 January 2025 238 1 View
I did not get any suspension cell adhesion protocol for immunofluorescence related studies. Any reference regarding this protocol will be utmost help. Thanks in advance.
05 January 2025 7,102 4 View
in various paper, i found that TNBC cell line constitutively express PD-L1. Kindly insight in this matter that whether interferone gamma administration is necessary or not?
01 January 2025 4,325 3 View
Suppose I want to perform MTT of suspension cells. In which I seed cells in colourless media supplemented with 10 % FBS. Now I cant discard media while adding MTT. In that case FBS is present...
24 November 2024 3,496 2 View
while addition of MTT; suppose media contain 10 % FBS. does it interfere with Final MTT reading at 570 nm or any kind of control should be taken for compensating that effect? K
22 November 2024 5,071 2 View
please insight into this topic
08 November 2024 7,237 2 View
Is it somehow related to tumour microenvironment or some other reason behind this.
08 November 2024 8,069 2 View
Is it ranging from 10000-20000 cells/ well in 96 well plate?
26 October 2024 852 1 View
kindly refer the protocol of that experiment
20 September 2024 7,885 2 View
Kindly mention the protocol
28 May 2024 3,309 0 View
kindly provide some insights
02 April 2024 4,739 2 View
