Hello Ananya,

Yes. Suspension cells may be adhered using poly-L-lysine coating.

For suspension cells you may follow the first few steps as provided below.

1. Prepare a 0.01% poly-L-lysine solution in Milli Q water.

2. Coat coverslips with excess poly-L-lysine (0.01% solution) for 10 minutes at room temperature.

3. Aspirate the excess and let the coverslips dry.

4. Rinse coverslips with water and place them in 6-, 12- or 24-well plates.

5. Wash cells with PBS and resuspend.

6. Add the resuspended cells onto the poly-L-lysine-coated coverslips and let them settle for 30–60 minutes.

7. Aspirate the excess cells followed by a gentle rinse with PBS.

Follow the next steps as usual (as per the standard immunofluorescence protocol) namely, fixation, permeabilization, blocking, incubation with primary antibody, incubation with the secondary and lastly mounting.

Alternatively, you could make use of Shi-fix coverslips. These are glass coverslips coated on both sides with Shi-fix biomaterial that enhances binding of non-adherent animal cells for imaging studies. Shi-fix coverslips allow suspension cells to be layered on them for imaging or cultured as a monolayer. These cover-slips are suitable for immunofluorescence and confocal microscopy. Please refer to the link below for more information about the product.

https://www.shikharbiotech.com/shi-fix-coverslips/

Best.

Thank you Malcolm Nobre

Dear Ananya Chatterjee

The technique for adherence of suspension cells involves coating of plastic or glass surfaces with charged substrates such as polyL-lysine to induce cellular attachment were found to require significant preparation time in the form of overnight treatment and well experimental planning [1]. Therefore, I recommend for you the protocol of Poly L lysine coating and related information for cell culture that well explained and covered in [2].

Best regards

[1] Article Protocol for Adhesion and Immunostaining of Lymphocytes and ...

[2] https://www.neuvitro.com/poly-l-lysine-coating-protocol

Dear Ananya,

A much better alternative to Poly-L Lysine coating is to use Shi-fix coverslips for suspension cell immobilization and immunostaining. the cell attachment is highly efficient, and there is virtually no cell loss during the immunostaining and washing procedures.

https://www.shikharbiotech.com/shi-fix-coverslips/

Best,

Ravindra