Hi everyone,Id like to ask if anyone had a protocol for ca+2 concentration measurement in HIT-T15 cells using Fura-2Am.Because I cannot find any suitable one.
Is there any free software for caring out these calculations.
21 October 2023 4,961 0 View
this will be done to knoe fibre composition
19 September 2023 8,199 2 View
Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....
13 September 2023 9,133 4 View
Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...
13 September 2023 4,587 1 View
Hi everybody, I have a questionnaire with 4 point Likert scale questions on participant experience. I want to score the results into low/moderate/high experience categories. How do I do this on...
15 June 2023 414 3 View
Please suggest any other method for estimating Heterogenous Panel Vector AutoRegressive thus allowing individual irf to be computed.
12 June 2023 9,061 3 View
Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...
17 April 2023 6,450 0 View
I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?
12 February 2023 7,826 0 View
The molecular weight of EDTA is 292.24g/mol Thanks in advance!
01 February 2023 5,421 3 View
I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using...
12 May 2022 786 1 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]
29 July 2024 6,626 0 View
Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...
17 July 2024 3,472 3 View
I am the leading author for the mentioned ID. I want to upload it but fail to do so.
18 June 2024 9,889 0 View
The website is https://researchgateway.in/award_reg?id=317 A friend came through this and asked me if this is authentic. When I researched, I found this is a market research company and giving...
14 June 2024 4,368 5 View
Each row is a therapy session by a variable label ID. Each unique ID has between 1 and 50 rows in SPSS (sessions). How can I select only those unique IDs with 3 or more sessions?
11 June 2024 6,036 5 View
please tell us my research gate id.
03 June 2024 3,623 0 View
Hello,
02 June 2024 9,328 1 View
Как найти свой Researcher ID?
30 May 2024 6,369 0 View
Hello. I can´'t find a full text article that i need for my research. Can you help me? The reference of the article is: Ref ID: 3441009. Article: Adolescent Health: Communication With Adolescent...
16 May 2024 3,293 3 View