I have been screening medicinal plants against Cryptococcus neoformans for approximately 1 year now. For about 9 months the H99 strain I have been using has been inhibited in my control column at concentrations between 25% and 12.5%. For the last 3 weeks the strain has been inhibited at much lower dilutions: 3.125%. What has happened to my strain? I must add that I have passaged from my glycerol stock 3 weeks ago after passaging from the previous culture for some time before. How is it possible that the yeast has now become so sensitive to acetone?
I suspect it may have been the parafilm I used in the last few screenings - the AeraSeal covers for 96 well plates I used before are probably more porous allowing for more gaseous exchange - resulting in better growth at higher acetone concentrations.
Hi , you can use Dimethyl sulfoxide (DMSO) is an organosulfur compound for prepared your extracts ...good luck .
I prepared my extracts in acetone.
Acetone works best - cryptococcus and many other microbes are least inhibited by acetone prepared extracts
Acetone and DMSO have similar inhibitory affects against fungi and yeast - also, acetone dissolves my plant extracts better than DMSO. The question was why the inhibition % had changed from 12.5% to 3.125%
See this link for discussion on different solvents
Yoram
https://www.researchgate.net/file.PostFileLoader.html?id=54fd5870d11b8ba67d8b4580&assetKey=AS%3A273728250810371%401442273308179
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