Did anybody ever attempt to combine a X-gal staining with an antibody staining ? We would like to try but if somebody else has done this and it works or not that would be very helpful.
I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...
11 February 2021 2,416 2 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...
06 January 2021 7,247 3 View
Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...
03 January 2021 1,010 6 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Many Universities have employees located across the world.
22 December 2020 6,380 3 View
I have been making a method to determine chlorhexidine using an LC-MSMS (ESI+ modus). My solutions are made in acetonitril/water (65/35) and 0,2% formic acid is added to ionize the compound. My...
14 December 2020 3,855 6 View
Is it true that only a very large sample is suitable for a panel data fixed effects estimation, or do the ols criteria apply?
26 November 2020 9,127 4 View
Hello all, Early next year I'm planning to evaluate a training for school principals. I will collect data from school principals with experience (what that entails is to be defined, but they...
13 November 2020 2,226 4 View
Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...
11 November 2020 3,582 6 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
24 February 2021 3,348 2 View
Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...
24 February 2021 9,578 4 View
Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...
24 February 2021 5,430 3 View
Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...
24 February 2021 9,902 5 View