Would there be weight loss in cancer patient having hypothyroidism ?

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What are the challenges and issues in opinion mining?

There is a huge popularity of community websites and social media sites across the internet of opinionated text on web. Users express their ideas and suggestions regarding various products and...

27 January 2020 9,336 2 View

Ferrite-polymer composites?

Hello I am facing problem in preparing ferrite-wax composites as the quantity of powder is very less (< 300 mg). If I melt the wax and mix ferrite using magnetic stirrer then ferrite stuck with...

22 May 2017 3,290 5 View

What is the incidence of huge ovarian tumours in Adolesent girls?

Rdeently I operated on a young girl of 16 yrs,with huge mass in the Abdomen.on CT scan revealed of Large ovarian tumour extending all over abdomen probably of neoplastic etiology.all tumour...

27 December 2014 4,207 3 View

Is anyone dealing with Metagenomic library construction from sludge DNA ?

I am to begin one such task and have decided on Bac as my cloning vehicle, and restriction digestion as the next step using BamH1 and pst1.

10 May 2012 3,295 4 View

PCR amplification enzyme

I am aiming to amplify a 1344 bp DNA region. Can you suggest me a good and high yielding Polymerase enzyme for the purpose? Also, the enzyme must be cost effective.

25 March 2012 2,224 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View