When preparing culture media for bacterial cultivation, Agar does not solidify properly with acidic pH, so, I need another alternative, pH-independent.
There are certain silica gels you can make, check the literature on bacterial remediation of acid mine drainage, these bacteria grow at extremely low pH and when people culture them they use different gels
Dear Sarhan..
Hope you would have got successive experiments with silica gel. If not, try to solidify the medium with electrophoresis-grade agarose (purified). It will work. Or try to use increased (double strengthen) dosage of agar agar, Type I.
Silica gel, pectin are other solidifying agent but i recommended to use highly purified agar which will work.
Pluronic polyol F127, a block copolymer of polypropylene oxide and ethylene oxide, may be used to solidify culture media for the enrichment, isolation and growth of micro-organisms. A stable gel is formed at temperatures above 10°C, the exact temperature depending on the concentration of polyol used. The most important feature of the polyol gel is that it liquefies as the temperature drops below the critical value for the concentration used. The gel may be autoclaved, and will alternate repeatedly between liquid and gel form. It does not appear to be toxic to aquatic bacteria, is more transparent than agar and may be used to isolate heat-sensitive organisms.
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