What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Can anyone help me troubleshoot QPCR for AAV titering?

So the lab that I work in generates a lot of AAVs and we titer them via QPCR. However, I've been noticing that the entire time, the efficiency has always been very low (anywhere from 40-80%) with...

22 November 2020 4,580 3 View

Can plant tissues be stored at -20C for DNA extraction?

Hey everyone. I collected some leaf tissue samples from the plant Phragmites australis from which I am hoping to extra DNA for sequencing. I will be extracting DNA using Qiagen DNeasy Plant Mini...

08 November 2020 4,989 12 View

Does anyone know of a marker expressed on all lymph-resident cells that can be used in flow cytometry?

I am looking for a marker found on all cells that reside in the lymph node - I study the immune response in salivary glands and we want to gate out any lymph resident cells to ensure that there is...

13 October 2020 6,322 2 View

Can you expect to see changes in secondary structure in a MD simulation?

While running a simulation on any peptide or protein, is it possible for the secondary structures to change? For example, seeing a steady rise in helical content over the whole simulation?

30 September 2020 4,298 9 View

What are ideal personal desktop hardware specs for comparing models for time series analysis and machine learning?

I am researching trading strategies, particularly involving time series analysis, but also machine learning, on data sets that range as far back as 1950 and would like to compare the accuracy of...

15 July 2020 7,733 3 View

How do I determine if my Fst values are significantly different from 0 or between populations that are and are not separated by a highway?

I am trying to determine if a highway is too much of a barrier for shrews to cross and breed freely. What I have to work with is ~1080 bp of the mitochondrial cytochrome b gene (we did not do...

22 June 2020 7,647 1 View

What is the best wearable EEG recording device for research?

Hi there, I am searching a wearable EEG device with as many channels and the highest accuracy possible. I found some papers which reviewed/used the following devices, but still not sure which is...

19 May 2020 5,712 2 View

Help needed with the anatomy of a mouse knee. What tissue is this?

Hi all, I have been collecting cross-sections of a mouse knee joint, and I keep seeing some differently coloured, yellowish tissue on the anterior side of the joint, but I'm not too sure what this...

27 January 2020 5,792 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View