We are hoping to decalcify mouse paws for IHC with EDTA.  However, with flesh still remaining around the paws, the decalcification has not been working (as determined by an ammonium oxalate test, presumably because the EDTA cannot access the bone through the soft tissue).  Since the phalanges (bone of interest) are much smaller than other bones for which decalcification is done, we are worried that trying to remove the flesh by hand will damage the bone. Is there an accepted way to remove flesh chemically without disturbing molecular structure for IHC or is careful physical removal the only option? Any advice appreciated!

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