Hello Ibukun Adesoji Adejumobi

You may use L-WRN cell line. It is available at ATCC (CRL-3276).

This cell line is a source for producing Wnt-3A, R-spondin 3, and noggin conditioned medium. The L-WRN cells were derived by transfecting L-Wnt3A cells with an R-spondin 3 and noggin co-expressing vector and stable clones were selected in medium containing G418 and Hygromycin B. The L-WRN cells secrete factors Wnt3A, R-spondin 3 and noggin into the medium.

Please refer to the link below.

https://www.atcc.org/products/crl-3276

Best.

A few cell lines can produce both WNT3A and R-spondin conditioned medium simultaneously. These include:

• L-WRN cell line (ATCC CRL-3276): This cell line is a mouse L cell line that has been engineered to secrete WNT3A, R-spondin-3, and Noggin. (Already mentioned by Dr. Malcolm)
• 3dGRO™ L-WRN Conditioned Media Supplement (Sigma-Aldrich): This product is a formulation based on DMEM/F-12 PLUS medium containing the 3 growth factors WNT3a (W), R-spondin-3 (R), and Noggin (N).
• 293T-HA-Rspol-Fc cell line (Trevigen): This cell line is a human 293T cell line that has been engineered to secrete R-spondin-1.

These cell lines can generate a conditioned medium rich in WNT3A and R-spondin, which is essential for the growth and maintenance of intestinal organoids.