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30 August 2020 2,230 5 View

How to get pure recombinant virus with plaque purification ?

We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...

01 August 2020 2,087 1 View

How to make Sentaurus silver.par to be the perfect conductor like defined electrode?

I have been trying to create some perfect conductor in my simulation as a connection between 2 spots(different doping semiconductor area). But is seems that the barrier bewteen silver and the...

10 March 2020 2,535 1 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

What is the best indoor spectrum that people need?

What is the best spectrum for an office, classroom, or other architectural scenes? Spectrum affects people's visual needs, work efficiency, comfort, psychological or physiological rhythm. How to...

20 December 2019 5,715 4 View

How can I remove RNA from IP samples without affecting protein?

09 October 2019 5,713 3 View

What effect does precipitation pH and heat treatment temperature have on the electrical resistance of the material?

I have prepared a series of nanomaterials by co-precipitation under different pH value and heat treatment temperature. I want to find a reasonable explanation about the change of the material...

21 August 2019 9,192 3 View

Sentaurus simulating anti-reflection coating issue?

I am starting to simulate the anti-reflection coating (ARC) for solar cells but I found some problems that I can't answer: 1, For solar cell without ARC, I tried to put the light source below...

03 July 2019 6,682 2 View

How to consider the question of theoretical support for statistical model choosing?

In my research I focus in paired correlated binary data. To accommodate the intraclass correlation, I choose to adopt a certain model without providing much theoretical support, and was criticized...

04 June 2019 7,887 12 View

does anybody have DR5::GUS vector to share?

does anybody have DR5::GUS vector to share? i need to check auxin in potato. thanks a lot.

03 March 2019 3,449 6 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View