With Nextera XT library prep, why low DNA yield on Fragment Analyzer when optimal tagmenation concentration of 0.2 ng/ul exceeded?

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Richard Ellis

Our experience is that the library yield is affected by the quality of the input DNA (presence of contaminants, etc) as much as by the concentration.

Ekaterina Stepanova

I am struggling with it as well. No matter I do, regardless additional purification steps with beads or columns, the yield is too low

I am working with DNA from fecal samples, thus, the purity of DNA might be an issue

Just tried to increase the number of cycles for the indexing step and got perfect results: try to go to 15 cycles instead of 12

Good luck!)