Does any know the difference between psPAX and PAX2 LV plasmid?

I want to know which one is more suitable for dendritic cell transduction. I see that in: https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6 PAX2 plasmid is used for dendritic cells...

31 December 2018 3,696 3 View

Is there a difference between human and mice codon optimised sequences?

I have a Cas9 plasmid which is human codon optimised. Will there be any difference in Cas9 protein expression if this is expressed in mice?

07 August 2018 5,808 0 View

How to add +/- signs in front of calculated mean value in Excel?

I'm not referring to error bars. I want to add +/- signs in front of my mean (just like in a paper), without having to go to insert symbols and inserting them individually. Are there any keys to...

10 November 2013 8,421 9 View

Is SOD and CAT activity present after 24h of autopsy provided its stored at -20C?

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09 October 2013 5,827 1 View

In which solvents are beta-carboline alkaloids soluble?

I tried it with water, 5% DMSO but it seems to insoluble in those solvents.

09 October 2013 6,216 7 View

Why are standards for phenolics dissolved in methanol for HPLC?

Why are standards for phenolics dissolved in methanol for HPLC?

09 October 2013 350 9 View

Identifing the epididymal luminal protein/s in lizards?

08 September 2013 2,651 4 View

TLC trouble shooting

I'm running TLC with silica sheets. As the solvent travels quarter way up its movement changes such that we no longer see a straight line of solvent but rather a squiggly line. This largely...

08 September 2013 7,048 15 View

What are your thoughts on my observations of STZ in fed and fasted conditions?

What should be the nutritional status of wistar rats before the injection of STZ.? I came across this very interesting paper titled 'Streptozotocin is equally diabetogenic whether administered to...

07 August 2013 6,918 1 View

what does 30% w/v of H2O2 mean?

what does 30% w/v of H2O2 mean? does it mean 30g of H2O2 is present in 100ml of that solution?

07 August 2013 529 17 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View