Can someone help me understand why we have obtained negative concentration values in our samples using an ELISA kit?

We have used a competitive immunoassay to determine the concentration of a neuropeptide in CSF samples. According to the literature, the levels of the neuropeptide should fit properly to the...

15 March 2020 6,568 3 View

No title

Hello, I don't understand why there is this error message on Amos... My data are from a survey and on SPSS everything is fine... Could you help me?

21 February 2020 3,001 3 View

Are we prepared for the consequences of climate change?

In places on the shores of the sea the tides will rise, the winter will be more icy and the summers will be hotter......

21 May 2018 5,041 11 View

Using model selection for a Phenotypic Selection Analysis?

Dear Fellow Researchgater's, I want to perform a phenotypic selection analysis on some floral traits using the classic method of Lande & Arnold (1983). For this analysis I estimated fitness...

01 February 2018 9,853 0 View

Are there any studies that explore the relationship between cuticle thickness and body size in species of decapod crustaceans?

This relationship is very obvious to me (think of lobsters), but hard to actually measure. I could not find any study which addresses this issue!

11 September 2017 1,995 14 View

Heat mediated antigen retrieval (using a microwave) for Olig2 immunohistochemistry staining?

11 January 2017 4,314 6 View

We are making a frozen stock, how much 20% glycerol should we add to 5ml spinned down overnight culture?

We need to grow 5ml of overnight culture according to the manufacturer. Then the protocol we have says to spin down the bacterial cells, but it doesn't mention the volume for reconstitution, is it...

30 November 2015 376 3 View

Do you know of any technique to separate chlorophylls from the plant extract?

The extracts are ethanolic and methanolic. I tried a partition with petroleum ether and hexane with very poor results. I need any faster technique than the column chromatography, The extracts are...

11 November 2015 9,531 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View