Are exisiting alternatives to chloro hydrate trypan blue decoloration?

More Laura Cuyàs Carrera's questions See All

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Is there a validated questionnaire for target audience research?

Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...

04 July 2024 9,001 0 View

Posting a research survey ?

Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...

30 June 2024 9,594 2 View

Can I use richer media to increase bacterial expression of human protein?

We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?

06 June 2024 6,071 1 View

How to scale up or down a restriction enzyme reaction?

Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...

28 May 2024 1,105 3 View

Algae contamination on hydroponic cultures for Arabidopsis thaliana?

Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...

21 May 2024 4,840 1 View

How to set up a restriction enzyme reaction?

I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...

19 May 2024 5,171 3 View

What is recommended as a strong and current reference of phenomenological analysis ?

What is recommended as a strong and current reference of qualitative phenomenological analysis ?

16 May 2024 2,142 1 View

Is there any guide to perform statistical analysis of transcriptomics?

I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)

09 May 2024 6,543 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

Can we find a statistical matrix mechanics equivalent to the Schrödinger equation?

We assume that we can find a statistical matrix mechanics equivalent to Schrödinger's PDE in two consecutive steps: i-Transform the Schrödinger PDE describing the wave function Ψ into its square...

27 July 2024 3,959 4 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Himanshu Tak

We are facing the same issue. Laura Cuyàs Carrera did you find any useful alternate to chloral hydrate for leaf clearing.

Laura Cuyàs Carrera

Finally we did not use trypan blue anymore, but we found an alternative to clear roots before stainning (three days incubation with K0H 20%). Hope it can be useful for you. Best regards.