I treat mice with IFA(100ug)+ protein of interest biweekly for 12 weeks and find their skin develops some kind of lesions and resulting in open wounds. Is it something common with adjuvants? How to avoid them and does it mislead my observation?
Hello Vivek, by biweekly, do you mean twice per week, or once every two weeks? That gives either 24, or 6 inoculations. If there is an immune response to your protein, then you will see inflammation at the later injection sites. Even if there is no immune response, IFA injections will still be an irritant, and giving mutliple injections over short time scales might play a part in an inflammatory response starting up. I would be inclined to scale back the number of inoculations, and/or try a different route that did not deposit in or under the skin (e.g. intraperitoneal). I am not sure that a different adjuvant will relieve the problem if it is associated with the number of injections (unless it allows you to reduce the number).
Best wishes,
David
Dear David, I do once every 2 week injection. I am thinking of MF59 as a better adjuvant for my work or even alum. I am wondering whether responses that i see with IFA would differ from other adjuvants. I would glad to know if there are some adjuvants that could only deliver the antigen but not elicit any immune responses which will enable me to conclude the observed response is due to my protein and not because of adjuvant.
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