Vivek Krishna P. Venu

12 Questions 17 Answers 0 Followers

Questions related from Vivek Krishna P. Venu

Fibrocytes

I am trying to look for circulating fibrocytes by FACS. Please advice on markers other than CD45 and intracellular collagen. Thanks Vivek

18 July 2017 914 4 View

Immunostaining for Smooth Muscle Cell Staining in Mice Colitis Tissues?

Have anyone succesfully performed Smooth muscle cell staining (actin or calponin) in DSS induced mice colitis models?

24 March 2017 8,941 0 View

BrdU Incorporation for Smooth muscle cell proliferation?

how much Brdu should be injected in mice to address smooth muscle proliferation like DSS induced Colitis?

09 March 2017 6,520 2 View

How to stain for Neutrophil Extracellular traps in paraffin embedded tissues?

Interested to address NET's in paraffin embedded mouse tissues.. Requesting suggestions for appropriate specific antigens.

03 September 2016 5,625 1 View

Is MyD88 or Trif mediated pathway is anti-inflammatory?

Interested to know, In TLR pathway, myd88 or Trif contribute to anti-inflammatory response? Thanks

20 August 2016 4,780 2 View

How to avoid Froth in Wire - Myograph upon incubation with catalase?

How to avoid Froth in Wire - Myograph upon incubation with catalase? I see many papers using 1200U of catalase which when used produces froth. Are there ways to overcome this? Thanks

18 May 2016 834 8 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

21 April 2016 7,880 14 View

How to address cholesterol efflux in primary residential macrophages?

How to address cholesterol efflux in primary residential macrophages? We can't access radioactivity so the classical approach couldn't be used. Any suggestions are welcome. Thanks Vivek

27 January 2016 456 2 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

22 January 2016 7,886 3 View

Will treating mice with Incomplete Freund's adjuvant cause skin lesions?

I treat mice with IFA(100ug)+ protein of interest biweekly for 12 weeks and find their skin develops some kind of lesions and resulting in open wounds. Is it something common with adjuvants? How...

11 December 2015 8,676 2 View

Is it possible to culture the bone marrow cells without differentiation?

I don't want them to get differentiated. i know they are stem cells, can i get them and culture as they are without getting them differentiated?

30 April 2015 9,078 3 View

To which extent are the use of murine aortic arch transplantation studies for atherosclerotic plaque regression valid?

Any video protocols available.

16 May 2014 7,106 1 View