I am using a three vectors system to package lentivirus in 293T cell lines. I have inserted GFP into my transfection vector. And I transfected all three vectors into 293T cell line with PEI and waited for supernatant collection. I want to know if I can check the transfection effect of PEI by detecting the GFP from 293T? Will the gene on the transfection vector also express during the lentivirus package?
You can quickly examine the PEI-mediated transfection of the GFP gene in a fluorescent microscope for the first component or you could use a GFP antibody after performing a western blot.
Yes you should observe some expression if you wait a few hours after transfection.
Yes, if the transfective vector from the lentivirus packaging system contains a functional GFP gene and is successfully transduced into the target cells, the GFP gene should express and result in the production of green fluorescent protein (GFP) in those cells. This expression can be used as a marker to indicate successful transduction and expression of the transgene in the target cells.
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