Venkat Ramana

4 Questions 6 Answers 0 Followers

Questions related from Venkat Ramana

Is there any way to study an ion and organic molecule interactions using computational methods??

I have a sensor which can show its sensing towards cu+ ion. I wanted to study their interaction computationally using some docking methods. Can any one help me in finding a way to do this.

08 July 2015 9,719 6 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

23 December 2014 3,121 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

18 July 2014 5,469 2 View

Can someone advise on the solubility of designed protein?

I tried many buffers in achieving my protein in soluble fraction, fortunately I am successful at pH1. Moreover, I have not used any buffer for pH1: its just milliq adjusted to pH1 using conc...

29 October 2013 5,462 1 View