Will increasing sugar concentration in a medium raise its acidity?

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Is it possible that molecular identification dont match with morpho-cultural based identification of fungi? How can I decide with its identification?

Good day everyone. I have isolated an endophyte. From its morphological characters it looks like Geotrichum sp. However, based on its ITS sequence it is a different species. It has 100% match with...

03 April 2018 6,554 11 View

Can I sterilize petroleum oil using an autoclave?

Im working on fungal oil degradation. My problem is sterilzation of diesel oil. How should I go about it?

08 September 2016 5,208 5 View

What fungal species is this?

I have isolated this from a vine. It seemed to produce VOC's as it inhibited growth of a test pathogen using double petri dish assay.

01 January 1970 5,992 18 View

What is the best way to incorporate petroleum oil in agar medium?

I'm screening for fungi that can degrade oil. What is the best way to add oil to agar medium? Thanks!

01 January 1970 4,621 0 View

Can fungi utilize carbon in vapor as carbon source?

Im doing an assay where I am testing if certain fungal species can metabolize diesel oil. Can I supply diesel oil in form of vapor? I'm planning to grow fungal isolates in mineral agar medium and...

01 January 1970 6,397 3 View

Is there a fast assay to determine if fungi emits volatile organic compounds?

I am looking for fungal endophytes that produce volatile organic compounds. Is there a fast assay, like a chemical compound that i can put on agar media, where the volatiles can react to (if the...

01 January 1970 5,769 5 View

Will shaking continuously increase growth of fungi in liquid medium?

I am working on fungal degradation of polyethylene. I have placed polyethylene powder in mineral salt medium and subsequently placed my fungal isolates. My question is do I really have to put them...

01 January 1970 4,192 6 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View