Is it possible that molecular identification dont match with morpho-cultural based identification of fungi? How can I decide with its identification?

Good day everyone. I have isolated an endophyte. From its morphological characters it looks like Geotrichum sp. However, based on its ITS sequence it is a different species. It has 100% match with...

03 April 2018 6,504 11 View

Can I sterilize petroleum oil using an autoclave?

Im working on fungal oil degradation. My problem is sterilzation of diesel oil. How should I go about it?

08 September 2016 5,072 5 View

What fungal species is this?

01 January 1970 5,947 18 View

What is the best way to incorporate petroleum oil in agar medium?

I'm screening for fungi that can degrade oil. What is the best way to add oil to agar medium? Thanks!

01 January 1970 4,579 0 View

Can fungi utilize carbon in vapor as carbon source?

Im doing an assay where I am testing if certain fungal species can metabolize diesel oil. Can I supply diesel oil in form of vapor? I'm planning to grow fungal isolates in mineral agar medium and...

01 January 1970 6,348 3 View

Is there a fast assay to determine if fungi emits volatile organic compounds?

I am looking for fungal endophytes that produce volatile organic compounds. Is there a fast assay, like a chemical compound that i can put on agar media, where the volatiles can react to (if the...

01 January 1970 5,721 5 View

Will shaking continuously increase growth of fungi in liquid medium?

01 January 1970 4,081 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View