Is it possible that molecular identification dont match with morpho-cultural based identification of fungi? How can I decide with its identification?

Good day everyone. I have isolated an endophyte. From its morphological characters it looks like Geotrichum sp. However, based on its ITS sequence it is a different species. It has 100% match with...

03 April 2018 6,504 11 View

Will increasing sugar concentration in a medium raise its acidity?

I will be looking at the effect of increasing sugar concentration (sucrose) on the volatile production of my fungal isolates. Will it also affect (decrease or increase) the pH of the growth medium?

31 December 2017 3,883 2 View

Can I sterilize petroleum oil using an autoclave?

Im working on fungal oil degradation. My problem is sterilzation of diesel oil. How should I go about it?

08 September 2016 5,072 5 View

What fungal species is this?

01 January 1970 5,947 18 View

What is the best way to incorporate petroleum oil in agar medium?

I'm screening for fungi that can degrade oil. What is the best way to add oil to agar medium? Thanks!

01 January 1970 4,579 0 View

Can fungi utilize carbon in vapor as carbon source?

Im doing an assay where I am testing if certain fungal species can metabolize diesel oil. Can I supply diesel oil in form of vapor? I'm planning to grow fungal isolates in mineral agar medium and...

01 January 1970 6,348 3 View

Will shaking continuously increase growth of fungi in liquid medium?

01 January 1970 4,081 6 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View