Is it possible that molecular identification dont match with morpho-cultural based identification of fungi? How can I decide with its identification?

Good day everyone. I have isolated an endophyte. From its morphological characters it looks like Geotrichum sp. However, based on its ITS sequence it is a different species. It has 100% match with...

03 April 2018 6,504 11 View

Will increasing sugar concentration in a medium raise its acidity?

I will be looking at the effect of increasing sugar concentration (sucrose) on the volatile production of my fungal isolates. Will it also affect (decrease or increase) the pH of the growth medium?

31 December 2017 3,883 2 View

Can I sterilize petroleum oil using an autoclave?

Im working on fungal oil degradation. My problem is sterilzation of diesel oil. How should I go about it?

08 September 2016 5,072 5 View

What fungal species is this?

01 January 1970 5,947 18 View

Can fungi utilize carbon in vapor as carbon source?

Im doing an assay where I am testing if certain fungal species can metabolize diesel oil. Can I supply diesel oil in form of vapor? I'm planning to grow fungal isolates in mineral agar medium and...

01 January 1970 6,348 3 View

Is there a fast assay to determine if fungi emits volatile organic compounds?

I am looking for fungal endophytes that produce volatile organic compounds. Is there a fast assay, like a chemical compound that i can put on agar media, where the volatiles can react to (if the...

01 January 1970 5,721 5 View

Will shaking continuously increase growth of fungi in liquid medium?

01 January 1970 4,081 6 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View