I am attempting to devise a preliminary cell based assay that concerns the impact of SASP factors on uptake of a particular extracellular factor. The assay of uptake already works, however it uses a cell line, specifically the H4 (https://www.atcc.org/products/all/HTB-148.aspx#documentation) glioblastoma line, which has deletions of PTEN and CDK2A.
Since these cells don't have mutations in p53, they should still be able to be induced into senescence via irradiation, but I'm unsure if the SASP pathways will be intact due to those deletions. If they would prevent normal SASP expression, is there any (neuronal) cell line that would be suitable, or are my only options primary cells/iPSC derived cells?
Based on the information you shared, it is likely that your cell line, when stimulated to induce senescence, would exhibit a SASP. However, the characteristics of the SASP are predicted to be unique to the cell line.
The answer largely depends on the definition of SASP you make.
If you consider just the up-regulation of inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha is sufficient to meet the SASP criteria, it is highly likely that SASP would occur.
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