09 September 2019 3 9K Report

Greetings,

I am performing a reverse transcription using the taqman microRNA assay protocol.

Parameters of thermocycler for this step are as follows: 30 min/16°C, then 30 min/42°C, then 5 min/85°C.

The program was only running for about 8 minutes, then the power failed for about 5 minutes before returning, and the program was restarted again.

How would that affect my reverse transcription product?

What would be the best course of action if such event occurred again?

Thank you.

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