Nada Moustafa

3 Questions 1 Answers 0 Followers

Questions related from Nada Moustafa

Will a power fail affect my cDNA synthesis?

Greetings, I am performing a reverse transcription using the taqman microRNA assay protocol. Parameters of thermocycler for this step are as follows: 30 min/16°C, then 30 min/42°C, then 5...

09 September 2019 9,457 3 View

Hippocampal tissue storage for miRNA analysis by real time PCR?

Greetings, We are planning to extract microRNAs from rat hippocampal tissue for quantification by real time PCR, we are using Qiagen miRNeasy extraction kits and the Taqman assays. I was advised...

07 July 2019 7,966 2 View

Which housekeeping gene and master mix to use in a brain microRNA level detection?

Greetings, We are conducting an experiment after which we will measure the levels of certain microRNAs in the hippocampi of albino rats. What would be the best housekeeping/reference gene to use...

02 February 2019 3,233 7 View