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Greetings, I am performing a reverse transcription using the taqman microRNA assay protocol. Parameters of thermocycler for this step are as follows: 30 min/16°C, then 30 min/42°C, then 5...
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Greetings, We are planning to extract microRNAs from rat hippocampal tissue for quantification by real time PCR, we are using Qiagen miRNeasy extraction kits and the Taqman assays. I was advised...
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Greetings, We are conducting an experiment after which we will measure the levels of certain microRNAs in the hippocampi of albino rats. What would be the best housekeeping/reference gene to use...
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