Does the size of the callus after infection increase the quantity of secondary metabolite?(Infection with Ti plasmid without a gene of interest)
in general sense infection of Ti plasmid cause increase of secondary metabolites.
There will be an increase in secondary metabolites as infection will result in some response by the plant callus. Are you trying to identify particular metabolites? Or have you transformed the callus to produce an enzyme for a particular metabolite?
Plants produce secondary metabolites as a response or as a part of a defensive mechanism whenever there is a stress. As for infection of Agrobacterium tumifaciens, certainly there will be production of secondary metabolites. But it is difficult to say about the amount of metabolites produced and especially quality metabolites produced out of this infection.In my opinion it is better you remove the vir genes or T-DNA and carry out the experiment. As regard to the details about metabolites produced here, you can refer the article and contact the author for further detail in this regard. Here is the reference: Production of plant secondarymetabolites: a historical perspective
F. Bourgaud, , A. Gravot, S. Milesi, E. Gontier
Laboratoire Agronomie et Environnement, UMR INRA-INPL-ENSAIA, 2 Avenue de la forêt de Haye, 54500, Vandoeuvre, France. http://dx.doi.org/10.1016/S0168-9452(01)00490-3,
There are reports suggesting that following Agrobacterium infection certain genes (upstream and down stream of T-DNA incertion) show over expression. This can result in the increased synthesis of certail metabolites. However it is not certain that Agrobacterium infection can result in the increased biosynthesis of sacendaryu metabolites
Under natural condition, if the plant is exposed to stress may be wounding, there is over production of secondary metabolites. During Agrobacterium infection under natural condition, a wound is created in some cells. If callus is formed from such injured cells, definately production of secondary metabolite irrespective of presence of T-DNA and vir. What is most important is wounding
Yes definitely an increase in the Sec met content could be expected in the transgenic cultures. The factors like Growth regulators, elicitors, A. rhizogenes/tumefaciens, culture age and varieng carbon, nitrate content can also cause the same result.
Yes sec metabolite production will be more due to callus injury at the time of co-cultivation and salycilic acid pathway and jasmonic acid pathway may enhance the sec met. Even acetosyrengone also enhance the sec met. Producing genes
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