Hi Wesley, I'm thinking probably not but if you could expand you're quesiton a bit perhaps we could have a better discussion.
Not necessary to repeat the assays with crude extracts (a complex mixture of many compounds and can mislead if the plant extract is obtained at different times as the free radical scavenging effect is closely related to chemical structures....polyphenols of many plats have good scavenging effect on free radical) you can better focus on isolated pure phyto-components of the same extract..
It is not necessary to do all the assays but to do some specific radical scavenging experiments e.g DPPH radical, OH radical scavenging and reducing power.
Hai Mr. Mark, Number of Free radical scavenging assays has been proposed so far for eg. DPPH, ABTS, FRAP, ORAC, OH, NITRIC OXIDE, SOD, METAL CHELATION, LIPID PEROXIDATION, H2O2, etc. I need to know, which assays involve same substrate (same radicals) in their reaction so that they cannot be repeated. Thank you......
Hi Servin Wesley, each assay that you cited involves differents free radicals, one of them involves an enzyme too (SOD). Depending of your interest in antioxidative activity you´ll choose them. For publications, reviewers not accept just one assay of antioxidant activity, it needs be confirmed by one or more different assays...
@Servin. None of the above assays use same substrate. You have to use different radicals for different assays. OH scavenging assays needs solvent evaporation if u are using ethanol as a solvent because sometimes ethanol acts as a OH scavenger.
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