Why would you use caffine to recover cells after UV irradiation?

More Shail Kabrawala's questions See All

Does oocyte generated from Oocyte like stem cell/neo-oogenesis/adult oogenesis is as good as natural oocytes?

Our long standing understanding for mammalian ovary is that it has fixed number of germ cells and duty of the ovary is to ovulate high quality ovum for successful fertilization and early embryonic...

11 October 2021 957 8 View

Similar to sperm funtion test, do we have scope to develop a oocyte function test for the management of women's egg factor issues?

As we know that the sperms function test is widely used for the management of male factor issues worldwide. However, similar parallel test is not available for the management of egg factor issue...

08 October 2021 4,336 2 View

Can the shape, number and distribution of mitochondria be used as a biomarker for oocyte quality?

Recent evidences suggest that mitochondria play important roles in determining the quality and fate of the oocytes in several mammalian species. Changes in shape, number as well as the...

05 October 2021 4,617 4 View

Which one is responsible for meiotic resumtion in mammalian oocytes i.e. "maturation promoting factor (MPF) activation or MPF destabilization?

The maturation promoting factor (MPF) is a key regulator of cell cycle that has regulatory subunit (Cyclins) and catalytic subunit (Cdks). Dissociation and degradation of specific cyclin...

29 September 2021 3,902 5 View

What are the latest advancements in computer-based SignLanguage Interpretation?

The Canadian Institute of Mass Communication (CIMCweb.com) is embarking on a pilot project to create language-barrier-free learning environment for deaf students, as a scalable model for...

28 July 2020 9,242 1 View

Is there any master switch that decides which death pathway will operate to eliminate a cell in mammalian tissue?

Type of insult (drugs, oxidants, intoxicants and other factors) and duration of exposure have been studies well to analyze the initiation of various death pathways (apoptosis, necrosis,...

13 June 2020 6,836 1 View

Can oocyte like cells generated through neo-oogensis undergo spontanous meiotic maturation in vitro?

Several studies indicate the presence of oogonial stem cells (OSCs) in postnatal mammalian ovaries. It has long been held that the ovaries contain a fixed number germ cells that remains...

05 June 2020 469 0 View

What structural differences enabled corina virus in rapid spread globally?

There are several pathogenic viruses but the way corona became pendemic remains mystry. Is it natural and came from animal origin or artificialy made so in the lab? Its spreading ability and...

18 May 2020 2,566 0 View

Does type and level of insult decide initiation of specific death pathway in mammalian cells?

Veriety of insult including stress initiate various death pathways in specific manner. It remains unclear whether specific type and its level decides which death pathway including PCD would be...

03 May 2020 3,672 0 View

Can we determine the fate of germ cells in mammalian ovary prior to ovulation?

Majority of female germ cells are eliminated from cohort of ovary and only few germ cells are culminated in oocyte and then ovum after ovulation.

01 May 2020 268 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Ireneusz Litwin

Caffeine is an inhibitor of ATM and ATR kinases so it prevents checkpoint activation.

Anne von Koschembahr

I agree with Ireneusz. Caffeine is known to inhibit ATM and ATR, which prevents the activation of checkpoint and the DNA damage response. What paper are you referring to? Do the authors state why they added caffeine following UV irradiation?

Daniel Vare

You could read this http://www.ncbi.nlm.nih.gov/pubmed/16968677 for more information effects of caffein following UV-irradiation