Hi anyone who reads this, I am a MRes student looking at antibiotic application on S. aureus biofilms. After disrupting my biofilm to quantify antibiotic application on TSA. I re exposed the same disrupted bacteria to TSB on a 96 well plate. The TSA plate quantified growth, whilst the TSB plate showed a lack of growth at some antibiotic concentrations that had grown on TSA. When re exposing the plated bacteria back to TSA there was a lack of growth.