Chelex vs. Glassmilk extraction approach for DNA sequencing?

Hi, I am looking into methods to extract DNA from fish tissue. Our lab has used the glassmilk approach in the past but recently switched to using Chelex. I was wondering if anyone knew of any...

24 September 2019 7,467 1 View

Is it possible to find the average of responses to a self-report scale?

My participants have rated themselves 1-10 on a series of 19 statements. Those statements are supposed to represent 5 different constructs. So for example, questions 2, 6 and 11 are all about...

06 February 2019 3,473 2 View

How long can PCR products labelled with FAM dye be stored before it begins to degrade?

I have PCR products stored at -20 degrees (Celsius) that are tagged with FAM dye. How long can these be kept before the fluorescent signal diminishes?

01 December 2018 2,081 3 View

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

01 November 2018 7,642 1 View

How to calculate protein interaction energy using VMD NAMD2?

I am using the VMD plugin QwikMD to do MD simulations of my protein-protein complexes. Hereafter I am using the NAMD2 analysis to calculate my total energy of the system along with all of the...

11 May 2018 1,481 5 View

What statistics should I used for Likert Scale?

Was wondering if anyone could help me, I am in my third year and I'm in dissertation hell. I have used 5 point Likert Scales in my questionnaire (Strongly agree, agree, so on). I'm now looking...

19 January 2018 6,122 5 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

Another question on transcription and coding, can anyone provide up to date help?

Hi everybody, I'm undertaking my first qualitative research and am somewhat overwhelmed by the (general consensus) of time it will take to transcribe qualitative interviews - I am intending on...

10 October 2017 3,011 3 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

What distinguishes the fourth industrial revolution from previous radical economic development eras ?

04 March 2016 6,069 11 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Is there research limitation on the relationships between digital capabilities, market orientation, brand perception and business performance?

I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...

14 February 2021 7,457 5 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

What could be an interesting topic for a bachelor's thesis in a finance/marketing/computer science field?

Hi everyone! I would like to write my bachelor's thesis on a topic that's currently relevant in the sphere of finance, marketing or computer science (or if it's possible a topic concerning all the...

08 February 2021 1,300 12 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

Which pump is used to transfer low melting temperature liquid metal from one container to another?

I want to transfer GaInSn alloy from one container to another with the help of an electromagnetic pump. Can anybody suggest me which brand or which company supplies these pumps?

25 January 2021 9,481 2 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Lupasol G100 from BASF -- where to buy?

Hello everyone. I am looking to purchase Lupasol G100 (essentially ~5kDa bPEI) for creation of carbon dots for in planta transient gene expression. Thus far, I have only been able to find a...

20 January 2021 7,683 1 View