Why we use non-fat or skim milk in blocking during ELISA and WESTERNBLOT , why it is only normal milk? what the science behind "non-fat"?

22 July 2022 1 5K Report

Why do we use non-fat or skim milk in blocking during ELISA and WESTERN BLOT, why it is only normal milk? what is the science behind "non-fat"?

Shehadeh Kaskous

The fat globules are known to block measurements in milk because they have a large volume in the liquid and sometimes cover other elements of the milk.

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