Why we don't use normal eppendorf during cryopreservation
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.
14 July 2024 3,120 7 View
need to know protocol for photoplate creation, styles, etc
11 July 2024 8,321 2 View
How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...
10 July 2024 5,040 8 View
I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.
08 July 2024 5,770 0 View
I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.
03 July 2024 4,044 3 View
Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India In my article, "Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India: Understanding Market Needs,...
03 July 2024 5,535 1 View
Please help me with one or more of the following, if possible: How does a client mentally process the trauma? What are the cognitive domains of processing? What measures are available to assess...
02 July 2024 6,263 0 View
I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...
02 July 2024 2,922 1 View
Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?
16 July 2024 9,576 1 View
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
I have cryopreserved the undifferentiated condition of SH-SY5Y cell line in the early passage in a cyomedia formulated with 90% FBS and 10% DMSO (sterile-filtered). Some months later, I have...
25 March 2024 1,152 2 View
Hi, I am currently looking for suggestions on choosing which cryovial to use for cell cryopreservation, internal or external threaded, do you guys have a preference? or is there any reason for...
11 March 2024 1,587 1 View
I know that CryoStore has a food and drug administration (FDA) master file to be use as cryopreservation medium but not sure is there are others? Anyone has an idea?
08 February 2024 3,955 2 View
I am running the CometAssay on cryopreserved avian blood samples. I had been obtaining what I thought were good results but then encountered a cloudiness problem the first time I tried to do more...
16 November 2023 8,530 0 View
How long we have to trypsinize A549 Cell as well as Beas2b cell line?? is it due to to fault during cryopreservation ?
28 October 2023 6,894 1 View
"Hello ResearchGate community! I'm a newcomer to the field of research and currently exploring potential research directions for my Ph.D. Among the options of cell cryopreservation, organoids, red...
22 October 2023 5,918 2 View
This week, a first vial of iPSC derived from hair follicles was thawed, using the same conditions as the last culture method, i.e. coating with Vitronectin and Essential 8 Flex as medium....
31 August 2023 7,027 2 View
Hi, the -80 freezer in my lab is down, it is only reaching -52C at the moment and I need to freeze down some cells. Can I put my vials in the CoolCell and place them at -20C or -50C and then...
24 August 2023 5,210 0 View
