What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

No title

11 January 2021 3,284 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

Why dose plasma AngII concentration decrease in AngII infusion mice detected by AngII ELISA kit?

I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...

31 December 2020 8,838 3 View

Is there a book that is of great benefit to a new research scholar working in the supply chain model?

I am talking about supply chain, so that the new research scholar will be of great benefit. After reading that, he was able to do a lot of improvement in his research.

31 December 2020 933 4 View

How can we count the number of edges in an edge detected image using canny/sobel algorithm in MATLAB?

I need to process an image for edge detection and then want to count the number of edges in that image.

29 December 2020 5,594 2 View

Cryopreservation of cancer cell lines @-80?

07 January 2021 5,410 8 View

For how long should a mammalian cell line be kept in -80 degree C after transferring it from liq nitrogen to culture the cell in media?

for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell

02 July 2020 9,912 1 View

How to prevent drying in 4% PFA fixed slides? What are some best techniques to store fixed slides for several weeks/months?

I am currently storing my fixed slides at 4 C. These slides tend to dry out. Are these slides still good to use? can these slides be revived by adding liquid? I have attached some images .

22 June 2020 7,070 5 View

Any reliable protocols for tissue detection of DNA damage with IHC or IF?

Dear all, Hope as a community your are doing fine. I have a question regarding antibodies against gH2AX, XRCC1 and p53bp1 that have worked for tissue detection for either IHC or IF, using...

08 June 2020 1,461 3 View

Does stored lc540 cells have negative effect when stored in liquid nitrogen?

I have stored my LC540 cells in liquid nitrogen 3 months back and wen I try to retreive cells die. Can anyone help me out. S soon as I take vial out from LN2 I place them in 37 deg C waterbath and...

05 June 2020 554 3 View

Can natural or organic products completely substitute for components of semen extender for cryopreservation?

Could an extender for semen cryopreservation be completely organic, owing to the growing interest for organic agriculture and safety concerns?

02 May 2020 3,696 1 View

What software should I use for cryobiologic image analysis?

I am a PhD student I am studying varying cryopreservation methods for sperm. As part of this project I have used Cryo-scanning electron microscopy to investigate varying ice-crystal formation...

16 April 2020 9,071 2 View

How long can mammalian cancer cell lines be stored in -80C?

I wanted to know if we can store frozen cells in -80 C refrigerator. These cells are stored in liquid nitrogen tank (-200 C) as of now. But as liquid nitrogen vaporizes, we need to keep refilling...

23 March 2020 8,753 10 View

Why we use FBS instead of culture media in cell freezing?

Hi, I have a simple question: When we freeze cells why we use 90% FBS and 10% DMSO? I know that DMSO is used for cell cryopreservation but I don't know why we use FBS instead of culture media?

28 January 2020 7,077 3 View

Why we use DMSO as cryopreservation agent in cell freezing despite its toxicity?

Hi, I know that we can use glycols instead of DMSO which can be less toxic but what are the advantages of DMSO over other cryopreservation agents that make us preferably use DMSO?

31 December 2019 8,303 3 View