Whilst many literature articles describe the use of Borate buffer (pH 8.5) for the PDL coating of cell culture surfaces the reasons why exactly seem a little harder to find. There are also plenty of protocols that just use sterile water as the solvent with, seemingly, equal success. The lysine amino group is fully protonated over a broad pH range so I'm guessing promoting protonation to aid interaction with the -vely charged plastic surfaces isn't the reason. I have found articles describing the antimicrobial properties of borate buffers so might be a reason but does anyone have a definitive answer.
Thank you.
I found this that may help:
https://www.neuvitro.com/polylysine-coating
Hello Marcus Hanley
Sodium borate buffer is a buffer that is used to maintain the pH within a relatively narrow range. It is isotonic and has a strong bactericidal effect. May be this could be the reason why it is used for coating procedures.
Best.
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