Hello dear Scientists, hope you all will be ok.
I have seen in many research articles that for extraction of phytochemicals from any plant, solvents i-e 70% Methanol or 95% Ethanol or any other solvent is used which is not 100% in its own pure form... So the question is that why dont people use pure Methanol or Ethanol as a solvent for the extraction? What is the logic behind using 70% or 90 or 95% Ethanol and not pure form the concerned solvent??
You will find (it is very common in liquid chromatography) that many molecules have both polar and nonpolar sidegroups. Thus, the best extraction solvent (or mobile phase) is a mixture that is both polar and nonpolar (water and an organic).
You will find (it is very common in liquid chromatography) that many molecules have both polar and nonpolar sidegroups. Thus, the best extraction solvent (or mobile phase) is a mixture that is both polar and nonpolar (water and an organic).
Hello,
Extraction procedure and solvent selection is only depend on your choice, when you use pure (100%) solvent that time only solvent soluble compounds can extracted from plant and if you use mixture of solvent in that condition you will get different compounds compared to pure solvent.
Good day!!
Hello,
As your sample contains both polar and non-polar components, use of a mixer of polar/non-polar solvent will give you best output. You also could use 100% of ethanol or methanol as they contain a polar end, however, mixer of water (polar) and alcohol (non/less polar) will help you to extract maximum compounds.
- 100% ethanol was less efficient to extract low molecular weight phenolic compounds with high antioxidant capacity as compared to 80% ethanol so we proceed with 70-80% ethanolic or methanolic extract
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