i try to understand the mechanism of TK in HSV and its important in genetics pathways
Hi !
I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program
10 November 2018 2,382 0 View
Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...
09 October 2018 6,052 0 View
this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out
31 December 2017 5,467 1 View
I have read a paper about plasmid and PCR then i found this Tm
31 December 2017 5,889 2 View
Writing a good review article is a real challenge could anyone tell me how to do that
11 December 2017 8,963 2 View
i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out
10 November 2017 6,148 6 View
I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid
10 November 2017 994 0 View
clone gene with BglII and EcoRI flanking into puc19
09 October 2017 2,730 4 View
If you don't have that experience or teacher to help you what should to do
09 October 2017 1,040 0 View
just i asking how i do that theoretically
09 October 2017 8,019 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...
20 July 2024 4,951 1 View