DNA was extracted from a traditional rice variety and those were confirmed and diluted prior to the pcr amplification.
PCR product confirmation was run at 125 V in the presence of 130 ml of 1X TBE in 1.5% agarose
Loaded PCR products volume - 3 microliters
I here attach the diluted DNA bands too
It looks like your pcr has failed and that also you added too much dna to the pcr reaction so you may just be looking at degraded dna and rna.If you run some of these pcr amplified samples alonside the amount of template dna which exists in your pcr mix and they may look very similar. It is important not to add too much dna in a pcr reaction as this can inhibit amplification. Try diluting 3 or your samples 1/2, 1/4 1/8 anf 1/16 and run all dilutions in the pcr and it may be that higher dilution samples amplify better. It is hard to troubleshoot pcr if you do not know how much actual dna you are amplifying and if your samples contain rna it is then more difficult to measure the amount that you are adding.Often 25-50ng total dna is plenty in a 50ul pcr reaction
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