Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

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30 November 2020 5,089 5 View

Is there any recommended sampling size for case study in qualitative techniques?

Recently, I have attended a training where the instructor said 36 is the recommended sample size of case study in qualitative techniques. I am curious to know if the total population is less than...

14 March 2020 4,566 4 View

Can I use IPA to study the perspectives of an in-group (eg; environmentalists) towards an outgroup (non-environmentalists)?

Can I use IPA to study the perspectives of an in-group (eg; environmentalists) towards an outgroup(non-environmentalists) rather than trying to find out the individual self experiences of in-group...

29 February 2020 5,789 5 View

How to calculate the Width of the diagonal strut of infill wall with openings located at different location say doors and window?

Width of the Equivalent diagonal strut of infill masonry wall with Openings located at different position. Is there any formulas or need to be done in FEM Software?

03 January 2020 1,030 1 View

How to choose a perfect glass for solar still?

I need to select a transparent glass for solar still. but i know the specification all. but i need high transmissivity glass. but i dont know what brand , and what thickness i should choose. i...

13 November 2019 9,799 4 View

What will be the suitable medium to do a corrosion test for a aerospace grade Ti alloy?

Please list out some common corrosion takes place in the aerospace components and suggest me the possible medium to perform corrosion test for the aerospace-grade Ti alloy in a lab. Applications...

25 September 2019 9,161 3 View

In Regression Discontinuity design,is it always necessary to assume participants above the threshold only to Treatment group & below to control grp?

Can't it be the other way? For an intervention to be provided in a educational study, it is good to assume the low scorers into the treatment grp so that they get benefitted more. but why is it...

21 July 2019 8,103 4 View

How is research design related with and different from research methodology??

How is research design related with and different from research methodology??

11 June 2019 4,449 3 View

How to extract eigenvalues of the stiffness matrix in Abaqus PDE?

I am trying to obtain the eigenvalues of the global stiffness matrix in abaqus. I am able to extract the stiffness matrix using the input file and use MATLAB to obtain the eigenvalues. But I want...

03 June 2019 1,829 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View