Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

Just face a Comsol issue dealing with magnetically hyperelastic material?

Hello all, I am just newly doing a simulation to test the deformation status of a magnetized rubber-like material, which is in-compressible, under a shifting magnetic field. Try to find the...

22 January 2021 7,427 2 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

NADP+/NADPH in bacterial cell lysates?

Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...

20 January 2021 232 2 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

Do anyone have experience with zymo research yeast plasmid miniprep?

Hello, I am new to yeast research. I am trying to rescue plasmid from yeast and have tried several protocols developed by researchers. But the results are not promising. I ended up buying this kit...

06 December 2020 1,251 2 View

How can I connect HC-05 to epoc x ( EEG ) sensor ?

Greetings , my name is Abdul and I have this research on how to move a module car by brain wave . The EEG sensor I have is emotive epoc x , I did the data analysis and am ready to move the car but...

11 January 2021 7,204 1 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

How can I classify Brain Waves on Python?

I'm working in my thesis project that's about how to avoid car accidents by body fatigue, I got some datasets where I got information abouth 12 person that had an EEG analisis when they were...

07 November 2020 4,845 2 View

What are the models of artificial intelligence based cars in automobile industry scientists are working currently with sustainable approach?

The question also includes the assurance of safety in the self-driving cars and the expected cost of selling. And what kind of quantum model will be more suitable in terms of physical realization?

29 October 2020 5,352 3 View

How can i limit the discharging energy from the EV in an Energy management system?

Hi everyone, i am making optimization for energy management system of EV charging station. I established the mathematical model objective function (minimization of electricity cost) and...

28 October 2020 7,267 3 View

To what extent do motorhomes and other large vehicles degrade road surfaces in relation to cars and other light vehicles?

I am studying the effects on the road system of a rural region of a drastic increase in traffic, particularly Motorhomes. My question is this, do motorhomes and other large vehicles have a...

22 October 2020 8,146 3 View

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV?

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV from cars of 100 employees (who come in the...

12 October 2020 4,720 3 View

Can you suggest topic of Mathematics for M.Sc. Thesis in Applied Mathematics ?

01 October 2020 5,677 12 View

Can a mixed background in mice reduce cre recombinase activity in comparison to a pure Bl6 background?

Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...

24 September 2020 1,792 3 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View