According to information from online database [MRTFA - Myocardin-related transcription factor A - Homo sapiens (Human) - MRTFA gene & protein](https://www.uniprot.org/uniprot/Q969V6) and [MKL1 protein expression summary - The Human Protein Atlas](https://www.proteinatlas.org/ENSG00000196588-MKL1), the molecular weight of MRTF-A (MKL 1) is 931 aa, 99 kDa.
However, the western blot predicted band size of antibodies ranged from 109 kDa (Anti-Mkl1/MRTFA antibody ab49311) to 145 kDa ([MKL1 Antibody 21166-1-AP | Proteintech](https://www.ptglab.com/products/MKL1-Antibody-21166-1-AP.htm) and [MKL1/MRTF-A Antibody | Cell Signaling Technology](https://www.cellsignal.co.uk/products/primary-antibodies/mkl1-mrtf-a-antibody/14760?N=0+4294956287&Nrpp=200&No=3400&fromPage=plp).
The predicted band size ranged from 109 kDa to 145 kDa. I am wondering the reason. How do I choose the appropriate MRTF-A antibody?
Thanks a lot!
Hi,
maybe this answers your question:
https://www.researchgate.net/post/Any_suggestion_on_why_different_predicted_band_sizes_appear_for_the_same_protein
Best regards
Hi there,
99kD is certainly deduced from the coding sequence. The apparent mobility on gel is not always strictly coincident with the theoritical MW (quite often proteins appear to be a bit bigger). Also it might be that your protein of interest is submitted to postranslational modifications which might affect the apparent mobility of the product on SDS/PAGE and therefore on WB. Further the primary transcript of the gene might also be subjected to alternative splicing which might results in the generation of differents isoforms of the protein. The 2 combined (splicing+modification) can generate a large diversity of products.
Check the source of target protein. which species (Human, Mouse, Rat) the antibody reacts with?
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