I just got one for five constructs, the other three can not be successful, and I can not find the problem. this method was reported as more than 90% positive. Is there anyone knowing why, please tell me, thank you!
Hello,
The Taq I used was Pfu Turbo Cx Hotstart DNA polymerase.
You should use Taq Polymerase for the proof reading when you are making any construct.
Pfu Turbo Cx Hotstart DNA polymerase is a proof reading polymerase.
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