We are maintaining Alternaria brassicae since 2005 on Alternate media. Initially on PDA, further Brassica leaf media and tomato extract media for maintaining Alternaria brassicae cultures after screening several media suitable for the growth of Alternaria brassicae. Now we obtained V8 juice online for maintaining Alternaria brassicae isolates in our laboratory. Carbon sources have been screened and sucrose was better than dextrose as per our studies. Now, we will be able to use V8 juice after suggestions of many researchers. Hope the conidial will be in original shape while growing on v8 juice media.