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After DNA ligation, I transformed into E. coli and get very small size colony but when I want to make a new LB plate by picking some colony, I did not get any colony culture. I incubated 37 temperatures 16 hr which resistant to kanamycin but it does no grown.
1) check if the antibiotic tat you use for the transformed E. coli is with the right concentration of antibiotic, are the plates freshly made?
2) When transferring colony, is the loop sufficiently cooled?
3) If there is no problem with the above 2 points, then u may need to redo the cloning
It sounds to me that those tiny colonies are not real, some strains of E. coli and can form micro-colonies on kanamycin plates which are not really resistant. Did you try to transform with a positive control plasmid that you know is Kan-resistant to see what those colonies look like?
Ayasha Akter did you have a negative control? Your colonies could be spontaneous mutants, check if you prepared your plates properly
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