10 February 2015 3 4K Report

Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??

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Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

Error of Reshape the data in Pythonplot code?

I am trying to plot using the attached file data file, "wan_band.dat". In the past, I also found the same error. But somehow it was resolved automatically. Again, I stuck with it. I am not able to...

09 April 2024 4,738 1 View

What are the diffrent nonpharmacological treatments mentioned in Ayurveda being in practices ?

List of Nonpharmacological treatments modules cost-effectiveness practices PMC publications

06 February 2024 9,814 2 View

HEK293T cells electroporation?

If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...

28 January 2024 8,008 0 View

Is blood isolated monocyte can be kept in incubator in which cancer cell lines are growing?

Dear researchers kindly let me know is it will be feasible to culture blood cells in animal cell culture lab and put the isolated cells to grow in cell culture medium in the same CO2 incubator in...

06 January 2024 1,189 5 View

How to prepare fecal slurry/water?

In our ongoing study we aim to assess the fecal microbiome and metabolome in patients in Indian population. For achieving the same we are referring to Franzosa Eric et al's., study entitled "Gut...

11 December 2023 8,754 0 View

Why no RNA formed?

I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....

19 November 2023 3,607 7 View

How shall I proceed with large plasmids?

What size of plasmid can be isolated from qiagen miniprep and midiprep kit, respectively? And at what temperature shall I grow a 36kb plasmid with 12.5ug/ml chloramphenicol resistance?

07 September 2023 2,600 3 View

Why no bands on Restriction Digestion of 36kb plasmid??

I am trying to isolate BAC plasmid of size 36kb. I tried with Qiagen Miniprep kit and Zymo BAC purification kit and ran on 0.5% gel. The bands can be seen on the gel but no bands were after I did...

31 August 2023 2,932 4 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

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